Phis2

http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf Webb29 okt. 2024 · To test whether CmMYB9a directly binds to the promoters of these four structural genes, yeast one-hybrid assays were performed with co-transformation of pGADT7 (AD)-CmMYB9a constructs and pHIS2 vectors containing the promoters. Co-transformation of the AD vector and these pHIS2 constructs served as negative controls.

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Webb16 feb. 2024 · The product was cloned into the pHIS2 vector for the bait construction. The coding sequence of PhOBF1 with full length was introduced into the pGADT7-Rec vector … Webb6 okt. 2024 · Furthermore, the AD-TFs and pHIS2- CmRAP2.3pro were co-expressed in Y187 in the SD/-His/-Leu/-Trp medium at 30°C for 3 days. Yeast cells harbouring AD vector and pHIS2-CmRAP2.3pro were used as the negative control. These yeast cells were incubated in SD/-His-Leu-Trp medium with 80 m m 3-AT at 30°C for 3 days. Luciferase … irish embassy passport tracker https://procisodigital.com

Transcription Factor-Centered Yeast One-Hybrid Assay

WebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. … Webb说明:文章内容仅供预览,部分内容可能不全,需要完整文档或者需要复制内容,请下载word后使用。下载word有问题请添加微信号:fanwen365或QQ:370150219 处理(尽可能给您提供完整文档),感谢您的支持与谅解。 WebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, … porsche tamiya 1/10

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Category:Y187-pHis2YeastOne-HybridMediaKit 使用说明书

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Phis2

pHis2载体质粒图谱、序列、说明书、价格_in - 搜狐

Webb1 feb. 2024 · To determine the optimal concentration of 3-amino-1, 2, 4-triazole (3-AT) to suppress background histidine leakiness of the pHIS2, three recombinant pHIS2 vectors … http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627

Phis2

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WebbSnapGene Viewer lets you see your data for free with the same rich visualization, annotation, and sharing capabilities as the fully enabled SnapGene software. SnapGene … WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community …

WebbpHIS-Hsv2 was a gift from James Hurley (Addgene plasmid # 42524 ; http://n2t.net/addgene:42524 ; RRID:Addgene_42524) For your References section: Two … WebbVector Map pGADT7-Rec Vector (Not sold separately) Sold as a part of Cat. Nos. 630490 & 630491 (102016) Page 2 of 4 . Figure 2. pGADT7-Rec Vector cloning site.

WebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251 http://www.labbase.net/Supply/SupplyItems-4229347.html

Webb10 aug. 2015 · The yeast one-hybrid assay was carried out using the Matchmaker one-hybrid system following the manual (Clontech, Palo Alto, CA, USA). The promoter …

Webb@phis2 a 2x2p2r w f2w 2x2sh. s 2 2 2 x ia 2 fi2 2 fpd2 fq2 2 fr2d2 fs2 2 ftd2 fu2 2 fvf pa x 2 2 2 2 d2 ! 2 #f qa p 2 2 ! 2 ! 2 2 % 2 & f ra 2 %2 2 ( & 2 ... irish embassy passportWebbOur dedication to customer support brings unique and effective solutions to your specific challenges and applications. Partnering with Takara Bio allows you to: Collaborate with … porsche targa 1972http://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825 irish embassy san franciscoWebb24 jan. 2024 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies … irish embassy sloveniaWebb27 mars 2015 · To suppress potential background activity, the medium was complemented with 50 or 100 m m 3-amino-1,2,4-triazole (3-AT) and yeast strains harbouring … irish embassy rabatWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … irish embassy tbilisiWebb20 dec. 2024 · GRAS transcription factor (TF) family is unique and numerous in higher plants with diverse functions that involving in plant growth and development processes, such as gibberellin (GA) signal transduction, root development, root nodule formation, and mycorrhiza formation. Walnut tree is exposed to various environmental stimulus that … porsche targa 1970