http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf Webb29 okt. 2024 · To test whether CmMYB9a directly binds to the promoters of these four structural genes, yeast one-hybrid assays were performed with co-transformation of pGADT7 (AD)-CmMYB9a constructs and pHIS2 vectors containing the promoters. Co-transformation of the AD vector and these pHIS2 constructs served as negative controls.
酵母杂交实验常见问题——单杂知识及自激活检测篇
Webb16 feb. 2024 · The product was cloned into the pHIS2 vector for the bait construction. The coding sequence of PhOBF1 with full length was introduced into the pGADT7-Rec vector … Webb6 okt. 2024 · Furthermore, the AD-TFs and pHIS2- CmRAP2.3pro were co-expressed in Y187 in the SD/-His/-Leu/-Trp medium at 30°C for 3 days. Yeast cells harbouring AD vector and pHIS2-CmRAP2.3pro were used as the negative control. These yeast cells were incubated in SD/-His-Leu-Trp medium with 80 m m 3-AT at 30°C for 3 days. Luciferase … irish embassy passport tracker
Transcription Factor-Centered Yeast One-Hybrid Assay
WebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. … Webb说明:文章内容仅供预览,部分内容可能不全,需要完整文档或者需要复制内容,请下载word后使用。下载word有问题请添加微信号:fanwen365或QQ:370150219 处理(尽可能给您提供完整文档),感谢您的支持与谅解。 WebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, … porsche tamiya 1/10