Phis2-p53

Webb12 apr. 2024 · PHIS2-p53+pGAD53m SD-TL SD-TLH 阳性对照 3AT是酵母HIS3蛋白合成的竞争性抑制剂，用于抑制His3基因的泄漏表达。 实验结果如下： 图1：背景筛选结果 结果 … WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: …

15 questions with answers in YEAST ONE-HYBRID Scientific …

WebbpBIND pACT-MyoD pBIND-Id pG5 luc pCMV-BD pCMV-AD pBD-p53 pBD-NF−κB pAD-SV40T pAD-TRAF pFR-luc pGBKT7 pGADT7 pCL1 pGBKT7-53 pGADT7-T pGBKT7-Lam pACT2 AD Y187 AH109 pSos pMyr pSos MAFB pHis2 pHisSi-1 p53blue pGAG424 pLaczi p53his pGAD53m pBridge Y2H Gold Yeast strain Y187 pIND WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … irc tin application form pdf https://procisodigital.com

核体系酵母单杂交文库服务-南京佰壹科生物技术有限公司

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb5 apr. 2024 · The Y187 strain co-expressing pGADT7-LkARF7 and pHIS2-LkBBM1 and the strain co-expressing pGADT7-LkARF19 and pHIS2-LkBBM1 grew normally on TDO medium containing 75 mM 3-AT, ... The bait vectors pGBKT7-p53 and pGBKT7-LaminC were co-transformed into AH109 with the prey vector pGADT7-LargeT as positive and negative … WebbThe corresponding transformation with pGADT7 prey vector containing WRKY18, WRKY40, or WRKY60 and pHIS2 bait vector containing p53 promoter fragment were used as negative controls. order cheesecake factory for pickup

p53-His,pLacZ,pHis，pHisi-1_MZ菌株_宁波明舟生物科技有限公 …

Category:酵母杂交实验常见问题——单杂知识及自激活检测篇

Tags:Phis2-p53

Phis2-p53

Yeast one-hybrid system—Matchmaker Gold - Takara Bio

Webb酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，. 产品型号：. 产品时间： 2015-11-08. 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯，pQE 载体，原核表达载体，克隆载体 ，毕赤酵母表达载体，毕赤 ... Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast …

Phis2-p53

Did you know?

Webb产品名称. p53-His,pLacZ,pHis，pHisi-1. 商品货号. B80888. 中文名称. 酵母单杂交. 冻干物说明. ·冻干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培养液或者无菌水溶解,接种 … Webbp53-His,pLacZ,pHis，pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。. 3、应用菌株 …

Webb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … http://www.labbase.net/Supply/SupplyItems-4229347.html

Webb24 jan. 2024 · The p53 fragment was from pGBKT7-p53 used for Y2H. ... The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. Webb14 aug. 2015 · zt028 酵母单杂交载体系统 pHis2 Clontech ... zt1220 pGBK-p53 . zt1221 pGC-silencer-U6-GFP . zt1222 pGIPZ . zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 ...

Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His …

http://www.bnbiotech.com/beinuobio-Products-11104093/ irc tin formWebb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。 将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ … order cheesecake factory online for pickuphttp://www.biofeng.com/zaiti/jiaomu/p53his.html order cheesecake factory onlinehttp://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825 irc tiny househttp://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf irc title 27 section 280.11Webb9 apr. 2024 · 目的：发现与A相互作用的motif 方法：以构建到pGADT7载体上的A基因为诱饵，筛选酵母单杂交motif文库，经过对阳性克隆的基因检测、DNA测序和比对分析，确定与pGADT7-A相互作用的motif。 材料： 1．诱饵克隆：pGADT7-A 2．诱饵载体：pGADT7 3．文库酵母质粒：motif 4．Clontech酵母单杂交系统 5．培养基 irc tiny homesWebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... irc to mp3