WebMay 1, 2024 · b, c, Relative mRNA expression of SLC7A11 (b) or IRF1 (c) in HT-1080 cells treated with IFNγ and JAK inhibitor I or ruxolitinib (0, 0.5 or 2 µM) for 24 h. d , ChIP of STAT1 in HT-1080 cells ... WebMay 9, 2024 · Antibodies were purchased from CST (normal rabbit immunoglobulin G [IgG] 2729, STAT3 12640) and Abcam (IRF1 ab26109). To calculate DNA enrichment in the ChIP assays, Real-time qPCR was …
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WebJan 21, 2024 · The eponymous member of the interferon regulatory factor (IRF) family, IRF1, was originally identified as a nuclear factor that binds and activates the promoters of type I interferon genes. However, subsequent studies using genetic knockouts or RNAi-mediated depletion of IRF1 provide a much broader view, linking IRF1 to a wide range of functions … CUT&RUN was performed with HeLa cells treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 4 hr) and IRF-1 (D5E4) XP ® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina ® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 5 (upper), including ERAP2 (lower), a known target gene of IRF-1 (see additional ... breath odor
(8478) IRF-1 (D5E4) XP® Rabbit mAb - CiteAb
WebNov 20, 2024 · Subsequently, equal samples quantified by DNA concentration were incubated with IRF1 antibody (D5E4) (CST, 8478) or ZNF350 polyclonal antibody (Abcam, ab77085) or IgG-negative control (CST, 2729) with 20 μL magnetic protein G beads overnight at 4 °C with rotation. After de-crosslinking and proteinase treatment, the … WebForm CT-1 (2024) Page . 2 Part II Record of Railroad Retirement Tax Liability Complete the Monthly Summary of Railroad Retirement Tax Liability below only if you were a WebNov 27, 2024 · RNA-seq data of IRF1 overexpressing piPSCs was uploaded to the NCBI Gene Expression Omnibus (GSE143484). Chromatin immunoprecipitation. Chromatin … breath ob/gyn/pediatrics hospital